Alternative model Danio rerio for screening evaluation of thermoand frigoprotective action of pharmacological compounds

Abstract

The introduction of bioethical principles into scientific biomedical research necessitates the search for and development of new alternative models, including those for screening potential pharmacological compounds. The main difficulty in developing alternative biomodels is the creation of a condition or pathology as close as possible to human ones both in terms of the mechanism of development of such a process and in terms of the response to pharmacological correction. Hydrobionts, possessing physiological reactions comparable to higher animals, have, among other things, organs and tissues similar in structure and functionality and have very great potential for screening studies and experimental pharmacology. They satisfy modern ethical concepts and norms limiting the scope of use of higher animals in experimental research. The article presents the results of work on the creation of an alternative model of Danio rerio for assessing the thermoand frigoprotective properties of pharmacological compounds. The possibility of modeling hypoand hyperthermia states in fish under laboratory conditions is demonstrated. A scoring system for assessing the condition of fish has been developed. Criteria for assessing hypoand hyperthermia are defined. Pharmacological validation of the developed model was conducted using reference drugs. The model's potential for screening the thermoand frigoprotective activity of pharmacological compounds was demonstrated.