The possibility of using echocardiography in small laboratory animals for acute pharmacological tests
https://doi.org/ 10.24411/2587-7836-2018-10015.
Abstract
Resume. The purpose of this investigation is to study the adequacy of the echocardiography method in small laboratory animals for performing acute pharmacological tests. In experiments on white mongrel male rats, a nonselective ß-adrenoreceptor agonist isoproterenol (20 μg /kg, i.v.) reduces the end-systolic and end-diastolic dimensions and volumes of the heart left ventricle, measured by echocardiography, and increases the shortening and ejection fractions, i.e. has a positive inotropic effect. Cardioselective ß1-adrenoblocker metoprolol (1 mg / kg, i.v.), on the contrary, increases the left ventricle sizes and reduces the shortening and ejection fractions, i.e. has a negative inotropic effect. Thus, the method of echocardiography in small laboratory animals can be used to conduct acute pharmacological tests.
About the Authors
E. O. Ionova
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
I. B. Tsorin
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
V. V. Barchukov
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
V. N. Stolyaruk
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
S. A. Kryzhanovskii
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
References
1. Boudia D., Domergue V., Mateo P., et al. Beneficial effects of exercise training in heart failure are lost in male diabetic rats. J. Appl Physiol. (1985). 2017 Dec 1;123(6):1579- 1591. DOI: 10.1152/japplphysiol.00117.2017
2. Squair JW, Liu J., Tetzlaff W., et al. Spinal cord injury-induced cardiomyocyte atrophy and impaired cardiac function are severity dependent. Exp Physiol. 2018 Feb 1;103(2):179- 189. DOI: 10.1113/EP086549
3. Wu LH, Zhang Q., Zhang S., et al. Effects of gene knockdown of CNP on ventricular remodeling after myocardial ischemia-reperfusion injury through NPRB/Cgmp signaling pathway in rats. J. Cell Biochem. 2018 Feb;119(2):1804- 1818. DOI: 10.1002/jcb.26341
4. Wang B., Ma X., Zhao L., et al. Injection of basic fibroblast growth factor together with adipose-derived stem cell transplantation: improved cardiac remodeling and function in myocardial infarction. Clin Exp Med. 2016 Nov;16(4):539- 550. DOI: 10.1007/s10238-015-0383-0
5. Yu B., Wang W. Cardioprotective effects of morroniside in rats following acute myocardial infarction. Inflammation. 2018 Mar;41(2):432-436. DOI: 10.1007/s10753-017-0699-x
6. Pessoa FG, Mady C., Fonseca KCB, et al. Erythropoietin reduces collagen deposition after myocardial infarction but does not improve cardiac function. Can J. Physiol Pharmacol. 2018 Jun;96(6):541- 549. DOI: 10.1139/cjpp-2017-0169
7. Shu L., Zhang W., Huang C., et al. Troxerutin protects against myocardial ischemia/reperfusion injury via pi3k/akt pathway in rats. Cell Physiol Biochem. 2017;44(5):1939- 1948. DOI: 10.1159/000485884
8. Rostamzadeh F., Najafipour H., Yeganeh-Hajahmadi M., Joukar S. Opioid receptors mediate inotropic and depressor effects of apelin in rats with 2K1C-induced chronic renovascular hypertension. Clin Exp Pharmacol Physiol. 2018 Feb;45(2):187- 197. DOI: 10.1111/1440-1681.12860
9. Tsorin IB, Palka IP, Chichkanov GG. Specific features of the effect of selective anxiolitic afobazole on cardiovascular system. Exp. Clin. Pharmacol. 2009;72(5):41- 45.
10. Lang RM, Bierig M., Devereux RB, et al. Recommendations for chamber quantification: a report from the American Society of Echocardiography’s Guidelines and Standards Committee and the Chamber Quantification Writing Group, developed in conjunction with the European Association of Echocardiography, a branch of the European Society of Cardiology. J. Am Soc Echocardiogr. 2005; 18( 12): 1440- 1463. DOI: 10.1016/j.echo.2005.10.005
11. Fowler NO, Holmes JC. Hemodynamic effects of isoproterenol and norepinephrine in acute cardiac tamponade. J. Clin Invest. 1969;48(3): 502- 507. DOI: 10.1172/JCI106007
12. Fehske W., Grube E., Steinborn W., et al. Cardiac effects of isoproterenol in the complex echocardiographic evaluation. Z. Kardiol. 1980;69(2):81-91.
13. Nigri A., Mangieri E., Martuscelli E., Reale A. Acute hemodynamic effects of acebutolol and propranolol. Clin Ther. 1984;6(5):693- 698.
14. Kalischer AL, Johnson LL, Johnson YE, et al. Effects of propranolol and timolol on left ventricular volumes during exercise in patients with coronary artery disease. J. Am Coll Cardiol. 1984;3(1):210-218.
Review
For citations:
Ionova E.O., Tsorin I.B., Barchukov V.V., Stolyaruk V.N., Kryzhanovskii S.A. The possibility of using echocardiography in small laboratory animals for acute pharmacological tests. Pharmacokinetics and Pharmacodynamics. 2018;(2):59-64. (In Russ.) https://doi.org/ 10.24411/2587-7836-2018-10015.
Views:
479
Email this article 