Effect of phenibut and atomoxetine on the biosynthesis and metabolism of dopamine and serotonin in the brain of C57BL/6 mice
https://doi.org/10.37489/2587-7836-2021-3-20-25
Abstract
The effect of intraperitoneal administration of the nootropic drug phenibut (70 mg/kg) and atomoxetine hydrochloride (3 mg/kg) on the neurochemical parameters of dopaminergic and serotonergic systems in the brain structures of C57BL/6 mice was studied by HPLC/ED. It was found that under in vivo blockade of L-aromatic amino acid decarboxylase (DAAA), both drugs in the selected doses did not affect directly on biosynthesis processes of both dopamine and serotonin in the prefrontal cortex and striatum of rodents. The observed effects of phenybut and atomoxetine hydrochloride, in comparison with the used D2 receptor ligands quinpirole (0.1 mg/kg) and sulpiride (25 mg/kg), suggest the absence of direct participation of dopamine autoreceptors regulating the functional activity of dopaminergic synapses.
Keywords
phenibut,
atomoxetine,
dopamine and serotonin biosynthesis,
prefrontal cortex,
striatum,
C57BL/6 mice,
HPLC/ED
Supporting agencies: The work was carried out within the framework of State task No. 0521-2019-0009 "Analysis of receptor mechanisms and search for pharmacological protection of the central nervous system in disorders of cerebral circulation and cognitive disorders".
About the Authors
N. N. Sukhorukova
FSBI “Zakusov Institute of Pharmacology”
Russian Federation
Russian Federation
Sukhorukova Natalia A. - Junior researcher, Laboratory of Radioisotope Research Methods
SPIN code: 2656-4174
Moscow
V. S. Kudrin
FSBI “Zakusov Institute of Pharmacology”
Russian Federation
Russian Federation
Kudrin Vladimir S. - PhD in Medicine, Head of the Laboratory of Neurochemical Pharmacology
SPIN code: 3986-3262
Moscow
V. B. Narkevich
FSBI “Zakusov Institute of Pharmacology”
Russian Federation
Russian Federation
Narkevich Victor B. - PhD in Medicine, Senior Research Scientist, Laboratory of Neurochemical Pharmacology
Moscow
G. I. Kovalev
FSBI “Zakusov Institute of Pharmacology”
Russian Federation
Russian Federation
Kovalev Georgy I. - Dr. Sci. (Med.), professor, Head of the laboratory
of radioisotope research methods
SPIN code: 8461-8814
Moscow
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For citations:
Sukhorukova N.N., Kudrin V.S., Narkevich V.B., Kovalev G.I. Effect of phenibut and atomoxetine on the biosynthesis and metabolism of dopamine and serotonin in the brain of C57BL/6 mice. Pharmacokinetics and Pharmacodynamics. 2021;(3):20-25. (In Russ.) https://doi.org/10.37489/2587-7836-2021-3-20-25
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