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Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC)

https://doi.org/10.24411/2587-7836-2019-10035

Abstract

Oxidative stress resulted to decrease in cells viability compared to the control (78 ± 5 % and 100 ± 6 %, respectively, р < 0,05). GK-2 and NGF prevented H2O2-induced HUVEC cells damages (95 ± 3 % for HA-2 and 97 ± 4 % for NGF vs. 78 ± 5 % for H2O2, p < 0.05). In control the number of cells with condensed chromatin was 9 ± 2, after the addition of H2O2 78 ± 9 (p < 0.05 compared with the control). The addition of NGF or GK-2 after H2O2 significantly reduced the number of such cells (44 ± 9 and 41 ± 8, respectively) (p < 0.05 compared to hydrogen peroxide) and prevented the development of morphological changes in nuclear chromatin. Thus GK-2, like NGF, in human endothelial cell culture HUVEC prevented the apoptosis development caused by hydrogen peroxide.

About the Authors

T. A. Antipova
FSBI «Zakusov Institute of Pharmacology»
Russian Federation


S. V. Nikolaev
FSBI «Zakusov Institute of Pharmacology»
Russian Federation


S. A. Kryzhanovsky
FSBI «Zakusov Institute of Pharmacology»
Russian Federation


E. S. Pekeldina
FSBI «Zakusov Institute of Pharmacology»
Russian Federation


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Review

For citations:


Antipova T.A., Nikolaev S.V., Kryzhanovsky S.A., Pekeldina E.S. Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC). Pharmacokinetics and Pharmacodynamics. 2019;(1):18-21. (In Russ.) https://doi.org/10.24411/2587-7836-2019-10035

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