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Quantitative determination of the content of venlafaxine and its active metabolite in human plasma using liquid chromatography-mass spectrometry

https://doi.org/10.37489/2587-7836-2022-4-26-32

Abstract

A validated method for the quantitative determination of venlafaxine, O-desmethylvenlafaxine in human plasma by tandem liquid chromatographymass spectrometry (HPLC/MS/MS) has been proposed. Sample preparation was carried out by liquid-phase extraction with support (SLE, from «supported liquid extraction») using HyperSEP cartridges and washing with 3-methylbutyl ether. The measure of the target analytes was carried on a triple quadrupole mass spectrometer under multiple-reaction monitoring (+MRM) mode with the electrospray ionization. The calibration curves were linear with regression coefficient r2 > 0.999. The method validation was showed high sensitivity, specificity, accuracy and reproducibility, as well as stability of analytes after freeze/thaw cycles and storage at –20 °C. The method is developed for using in therapeutic drug monitoring.

About the Authors

I. I. Kuzmin
FSBSI «Mental Health Research Center»
Russian Federation

Kuzmin Ivan I., Research assistant of laboratory of evidence-based medicine and biostatistics

SPIN code: 1442-0325

Moscow



A. I. Platova
FSBSI «Mental Health Research Center»
Russian Federation

Platova Angelina I., Research assistant of pharmacokinetics laboratory

SPIN code: 6656-2194

Moscow



A. S. Konstantinova
LABINVEST LLC
Russian Federation

Konstantinova Anastasiya S., Service Engineer

Moscow



I. I. Miroshnichenko
FSBSI «Mental Health Research Center»
Russian Federation

Miroshnichenko Igor I., Dr. Sci. (Med.), Head of pharmacokinetics laboratory

SPIN code: 4117-9703

Moscow



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Review

For citations:


Kuzmin I.I., Platova A.I., Konstantinova A.S., Miroshnichenko I.I. Quantitative determination of the content of venlafaxine and its active metabolite in human plasma using liquid chromatography-mass spectrometry. Pharmacokinetics and Pharmacodynamics. 2022;(4):26-32. (In Russ.) https://doi.org/10.37489/2587-7836-2022-4-26-32

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ISSN 2587-7836 (Print)
ISSN 2686-8830 (Online)