Quantification of a potential anticonvulsant drug GIZh-298 in rat plasma by liquid chromatography-mass spectrometry
https://doi.org/10.37489/2587-7836-2022-3-37-45
Abstract
GIZh-298 is a new derivative of 4-phenylpyrrolidone with anticonvulsant and nootropic effects. A selective and sensitive HPLC-MS technique for the quantitative determination of GIZh-298 in rat blood plasma has been developed and validated. The linearity of the technique was confirmed by a high correlation coefficient (>0.99). Recovery of GIZh-298 from blood plasma averaged 73.4 ±3.4 %. Accuracy during one working cycle and between cycles was < 4.01%, precision < 8.03 %. The study of the stability of GIZh-298 revealed that the target compound is stable in a biomatrix at room temperature (4 h), when it is in a thermostatically controlled autosampler (8 °C) during an analytical experiment, with prolonged storage at -50 ° C for 30 days, and also if it is subjected to several freeze-thaw cycles (3 cycle). The pharmacokinetics of GIZh-298 in rat blood plasma after a single intravenous injection at a dose of 60 mg/kg was studied.
About the Authors
P. O. Bochkov
FSBI «Zakusov Insti¬tute of Pharmacology»
Russian Federation
Russian Federation
Bochkov Pavel O. PhD, Cand. Sci. (Biology), Senior Research Officer of laboratory pharmacokinetics
SPIN code: 5576-8174
Moscow
O. Yu. Kravtsova
FSBI «Zakusov Insti¬tute of Pharmacology»
Russian Federation
Russian Federation
Kravtsova Oxana Yu. PhD, Cand. Sci. (Biology), researcher scientist of pharmacokinetics laboratory
SPIN code: 1733-2330
Moscow
G. B. Kolyvanov
FSBI «Zakusov Insti¬tute of Pharmacology»
Russian Federation
Russian Federation
Kolyvanov Gennadiy B. Dr. Sci. in Biology, Leading researcher of the laboratory of pharmacokinetics
SPIN code: 2538-8639
Moscow
A. A. Litvin
FSBI «Zakusov Insti¬tute of Pharmacology»
Russian Federation
Russian Federation
Litvin Alexander A. Dr. Sci. in Biology, Leading researcher of the laboratory of pharmacokinetics
SPIN code: 6193-5770
Moscow
S. S. Boyko
FSBI «Zakusov Insti¬tute of Pharmacology»
Russian Federation
Russian Federation
Boyko Svetlana S. PhD, Cand. Sci. (Biology), research officer of laboratory pharmacokinetics
SPIN code: 4176-8921
Moscow
V. P. Zherdev
FSBI «Zakusov Insti¬tute of Pharmacology»
Russian Federation
Russian Federation
Zherdev Vladimir P. Dr. Sci. (Med.), professor, Head of laboratory pharmacokinetics
SPIN code: 2213-9592
Moscow
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Review
For citations:
Bochkov P.O., Kravtsova O.Yu., Kolyvanov G.B., Litvin A.A., Boyko S.S., Zherdev V.P. Quantification of a potential anticonvulsant drug GIZh-298 in rat plasma by liquid chromatography-mass spectrometry. Pharmacokinetics and Pharmacodynamics. 2022;(3):37-45. (In Russ.) https://doi.org/10.37489/2587-7836-2022-3-37-45
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