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Noopept protects ciliates Paramecium сaudatum cells from the toxic effects of heavy metal compounds

https://doi.org/10.37489/2587-7836-2022-1-9-13

Abstract

   Background. The dipeptide drug Noopept, created at the Zakusov Institute of Pharmacology, has a wide range of pharmacological actions, including cytoprotective one. In connection with the increasing pollution of the environment, it is urgent to identify the protective properties of drugs with a negative effect of heavy metal compounds on the live system.

   The aim of the present study was to investigate the cytoprotective effects of Noopept under toxicity induced by heavy metal compounds using ciliates Paramecium caudatum as a test object.

   Methods. The ciliates Paramecium caudatum were exposed to 4 salts of heavy metals: cadmium chloride, lead acetate, copper sulfate, zinc sulfate, as well as nanoparticles of copper oxide and zinc oxide. Noopept was added to the medium with the experimental cells 30 min before the introduction of damaging agents, at concentrations of 0.01–10 μM. Results. It was found that the presence of metal salts in the medium significantly reduced the number Paramecium сaudatum cells, depending on the time of exposure. Noopept at all studied concentrations reduced cell death, while the maximum intensity of the effect was observed at a concentration of 1 μM.

   Conclusions. The results obtained make it possible to supplement data on the wide spectrum of Noopept's action.

About the Authors

O. V. Karpukhina
Federal State Educational Institution of Higher Professional Education Lomonosov Moscow State University; Russian Academy of Sciences
Russian Federation

Olga V. Karpukhina, PhD Biological Sci., Leading researcher

N. N. Semenov Federal Research Center for Chemical Physics

Faculty of Biology

Department of higher nervous activity

SPIN code: 6762-7087

Moscow



P. Yu. Povarnina
FSBI “Zakusov Institute of Pharmacology”
Russian Federation

Polina Yu. Povarnina, PhD Biological Sci., Senior research scientist

Department of medicinal chemistry

Laboratory of peptide bioregulators

SPIN code: 5498-6724

Moscow



A. N. Inozemtsev
Federal State Educational Institution of Higher Professional Education Lomonosov Moscow State University
Russian Federation

Anatoli N. Inozemtsev, Dr. Sci. (Biol.), Leading researcher

Faculty of Biology

Department of higher nervous activity

SPIN code: 7119-9015

Moscow



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Review

For citations:


Karpukhina O.V., Povarnina P.Yu., Inozemtsev A.N. Noopept protects ciliates Paramecium сaudatum cells from the toxic effects of heavy metal compounds. Pharmacokinetics and Pharmacodynamics. 2022;(1):9-13. (In Russ.) https://doi.org/10.37489/2587-7836-2022-1-9-13

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