HPLC-MS method development and validation for simultaneous quantitation of GZK-111 and CPG compounds in rat plasma
A. L. Podolko,
P. O. Bochkov,
G. B. Kolyvanov,
A. A. Litvin,
O. G. Gribakina,
R. V. Shevchenko,
V. P. Zherdev https://doi.org/10.37489/2587-7836-2019-4-28-36
Abstract
The technique of quantitative determination of GZK-111 and CPG compounds in rat blood plasma has been developed. The analysis was carried out using the combined method of high-performance liquid chromatography with mass spectrometric detection. The method was linear in the concentration range of 25-1000 ng/ml for both compounds. Percentage of GZK-111 extraction from rat blood plasma was 57.8 %. The lower limit of detection for GZK- 111 compound was 25 ng/ml. Percentage of extraction of CPG compound was 42.5 %. The lower limit of detection of CPG compound was also 25 ng/ml.
About the Authors
A. L. Podolko
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
Podolko Anna, SPIN code: 4707-3068, Junior Research Officer of laboratory pharmacokinetics, Moscow
P. O. Bochkov
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
Bochkov Pavel, SPIN code: 5576-8174, Candidate of Biological Sciences, Senior Research Officer of laboratory pharmacokinetics, Moscow
G. B. Kolyvanov
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
Kolyvanov Gennadiy, SPIN code: 2538-8639, Doctor of Biological Sciences, Leading researcher of the laboratory of pharmacokinetics, Moscow
A. A. Litvin
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
Litvin Alexander, SPIN code: 6193-5770, Doctor of Biological Sciences, leading researcher of the laboratory of pharmacokinetics, Moscow
O. G. Gribakina
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
Gribakina Oxana, SPIN code: 6266-8161, Candidate of Biological Sciences, Research Officer of laboratory pharmacokinetics, Moscow
R. V. Shevchenko
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Russian Federation
Shevchenko Roman, SPIN code: 1844-6202, Candidate of Medical Sciences, Research Officer of laboratory pharmacokinetics, Moscow
V. P. Zherdev
FSBI «Zakusov Institute of Pharmacology»
Russian Federation
Zherdev Vladimir, SPIN code: 2213-9592, Doctor of Medical Sciences, professor, Head of laboratory pharmacoki-netics, Moscow
Russian Federation
Zherdev Vladimir, SPIN code: 2213-9592, Doctor of Medical Sciences, professor, Head of laboratory pharmacoki-netics, Moscow
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Review
For citations:
Podolko A.L., Bochkov P.O., Kolyvanov G.B., Litvin A.A., Gribakina O.G., Shevchenko R.V., Zherdev V.P. HPLC-MS method development and validation for simultaneous quantitation of GZK-111 and CPG compounds in rat plasma. Pharmacokinetics and Pharmacodynamics. 2019;(4):28-36. (In Russ.) https://doi.org/10.37489/2587-7836-2019-4-28-36
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