phkineticaФармакокинетика и ФармакодинамикаPharmacokinetics and Pharmacodynamics2587-78362686-8830ООО «Издательство ОКИ»10.24411/2587-7836-2018-10004.phkinetica-50Research ArticleДОКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ ФАРМАКОДИНАМИКИPRECLINICAL PHARMACODYNAMICS STUDIESИзучение ангиотропной активности фактора роста нервов в опытах на культуре эндотелиальных клеток человека (HUVEC) Теравит Антистресс и его компонентовStudy of angiotropic activity of the nerve growth factor in experiments on human endothelial cell culture (HUVEC)ПекельдинаЕвгения СергеевнаPekeldinaE. S.noemail@neicon.ruНиколаевСергей ВладимировичNicolaevS. V.noemail@neicon.ruАнтиповаТатьяна АлексеевнаAntipovaT. A.noemail@neicon.ruКрыжановскийСергей АлександровичKryzhanovskiiS. A.SAK-538@yandex.ruФГБНУ «Научно-исследовательский институт фармакологии имени В.В. Закусова»РоссияFSBI «Zakusov Institute of Pharmacology»Russian Federation201806022018013235Copyright © Пекельдина Е.С., Николаев С.В., Антипова Т.А., Крыжановский С.А., 20182018Пекельдина Е.С., Николаев С.В., Антипова Т.А., Крыжановский С.А.Pekeldina E.S., Nicolaev S.V., Antipova T.A., Kryzhanovskii S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmacokinetica.ru/jour/article/view/50

В экспериментах in vitro, выполненных на культуре клеток эндотелия человека (HUVEC), изучали ангиотропные эффекты фактора роста нервов NGF. На интактной культуре клеток показано, что NGF в концентрации 10-9 M проявляет ангиогенные свойства и стимулирует начальную стадию ангиогенеза - тубулогенез, о чём свидетельствует статистически значимое по сравнению с контролем увеличение средней суммарной длины микротрубочек. В условиях оксидативного стресса NGF нивелирует повреждающее действие перекиси водорода в высокой концентрации (200 мкМ) и не только проявляет ангиопротекторное действие, но и препятствует развитию морфологических изменений ядерного хроматина, характерных для апоптоза. В этих условиях NGF (10-9 М) статистически значимо по сравнению с контролем снижает число клеток с конденсированным и фрагментированным хроматином: соответственно 44 ± 9 и 78 ± 9 (р ≤ 0,05). Полученные в работе результаты подтверждают данные о наличии у NGF ангиогенной активности и свидетельствуют о том, что в условиях повреждения эндотелиальных клеток сосудов оксидативным стрессом он проявляет выраженную ангиопротекторную и антиапоптотическую активность.

Resume. In vitro experiments performed on human endothelial ceLL culture (HUVEC), nerve growth factor (NGF) angiotropic effects were studied. An intact ceLL culture it was shown that NGF exhibited angiogenic properties and stimulated the initial stage of angiogenesis -tubuLogenesis at a 10-9 M concentration, as evidenced by a statisticaLLy significant increase in the average totaL Length of microtubuLes compared with the control. Under conditions of oxidative stress, NGF neutraLizes the damaging effect of hydrogen peroxide in a high concentration (200 |±M) and not onLy shows an angioprotective effect, but aLso prevents the deveLopment of morphoLogicaL changes in nucLear chromatin, characteristic for apoptosis. Under these conditions NGF (10-9 M) reduces statisticaLLy significant by comparison with the controL the number of ceLLs with condensed and fragmented chromatin: 44 ± 9 and 78 ± 9 (р ≤ 0,05), respectiveLy. The obtained resuLts confirm the data about the NGF angiogenic activity and indicate that under conditions of the vesseLs endotheLiaL ceLLs damage by oxidative stress, it exhibits pronounced angioprotective and anti-apoptotic activity.

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The authors declare that there are no conflicts of interest present.