phkineticaФармакокинетика и ФармакодинамикаPharmacokinetics and Pharmacodynamics2587-78362686-8830ООО «Издательство ОКИ»phkinetica-147Research ArticleАКТУАЛЬНЫЕ ОБЗОРЫCURRENT REVIEWSОбзор существующих методик оценки активности CYP2D6 с применением экзогенных и эндогенных маркеровReview of existing methodologies to assess the activity of CYP2D6 using exogenous and endogenous markersАбдрашитовРустем ХамзиевичAbdrashitovA. D.rustixxx13@gmail.comГильдееваГ. Н.GildeevaG. N.noemail@neicon.ruРаменскаяГ. В.RamenskayaG. V.noemail@neicon.ruСмирновВ. В.SmirnovV. V.noemail@neicon.ruГБОУ ВПО «Первый Московский Государственный Медицинский Университет им. И.М. Сеченова»РоссияFirst Moscow State Medical University named after I.M. SechenovRussian Federation20150904201401411Copyright © Абдрашитов Р.Х., Гильдеева Г.Н., Раменская Г.В., Смирнов В.В., 20142014Абдрашитов Р.Х., Гильдеева Г.Н., Раменская Г.В., Смирнов В.В.Abdrashitov A.D., Gildeeva G.N., Ramenskaya G.V., Smirnov V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmacokinetica.ru/jour/article/view/147

Приведены особенности функционирования и полиморфизма изофермента цитохрома Р450 CYP2D6. Рассмотрены существующие методики определения его активности при помощи эндогенных маркеров. Представлен обзор исследований по скринингу эндогенных субстратов, подвергающихся биотрансформации преимущественно под воздействием изофермента CYP2D6. Изложены результаты по определению отношения пинолина к его метаболиту 6-гидрокси-1,2,3,4-тетрагидро-бета-карболину in vitro на культуре клеток и in vivo на мышах для последующей оценки активности изофермента CYP2D6.

Peculiarities of functioning and polymorphism of cytochrome P450 isoenzyme CYP2D6. Existing methods for determining its activity using endogenous markers. A review of studies on the screening of endogenous substrates, biotransformation mainly under the influence of isoenzyme CYP2D6. The results by determination pinoline relationship to its metabolite 6-hydroxy-1,2,3,4-tetrahydro-beta-carboline in vitro in cell culture and in vivo in mice for evaluation isoenzyme activity CYP2D6.

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The authors declare that there are no conflicts of interest present.